Collections are a way of saving curated lists of annotated sequences, complete with assay data and other relevant sequence information. Collections can be searched against to find any sequences that are closely related to your selected candidates.
There are three ways to add sequences to a Collection:
- Adding sequences when the Collection is created
- Adding and annotating new, unannotated sequences from the Collections page (this article)
- Importing chosen sequences from an existing Annotator Result document
Once added, sequences in a Collection are assumed to have been annotated with the relevant antibody regions.
Adding new unannotated sequences
Sequences can be added to a Collection directly via the Add Sequences button in the Collection's Sequences Table.
Adding sequences from within the Collection works the same as adding sequences during the creation of a Collection. You can add up to 1000 standard antibody sequences at a time.
1. Add Sequences
Sequences and sequence lists can be selected for import in the Sequence Import options dialog.
This file tree will only show the sequence types that match the Collection. For example if the chosen Collection is Nucleotide type, then only Nucleotide sequences and sequence lists will be shown.
This step uses the Antibody Annotator pipeline to annotate the selected sequences as all sequences in a Collection are assumed to be annotated. Refer to the Antibody Annotator help article for this step.
Tip: If you usually use a different Annotator than Antibody Annotator, you can import sequences directly from your Annotator result instead.
The Summary step shows a brief summary of information from the previous steps. From here you can go back to change your settings, or click the Run button to begin adding sequences to your Collection.
Once the Add Sequences job is complete, your new sequences will appear at the bottom of your chosen collection.
Collections can be searched from within Antibody Annotator, Single Clone, and Scaffold Annotator Results. For more information about searching for sequence matches, see the Searching a Collection article.